Carrington

Mary N. Carrington, Ph.D.

Lab Info:

Principal Investigator: Mary N. Carrington, Ph.D.

Lab Staff: 4 scientists, 4 technicians, 2 postdocs

Office/Location: Frederick, MD

Phone: (301) 846-1390

Email: carringm@mail.nih.gov

Category: Steering Committee

Dr. Carrington is a Senior Principal Scientist within the Frederick National Laboratory and head of the HLA Immunogenetics Section in the Laboratory of Integrative Cancer Immunology and a Steering Committee Member at the Ragon Institute. Her group studies the influence of genetic variation on risk of human disease, outcome to therapeutic treatment, and vaccination.

 

The human leukocyte antigen (HLA) class I and class II genetic loci are the main focus of Dr. Carrington’s research. While HLA allele disease associations are often attributed to variation at positions that determine specificity for antigenic peptides, there are other mechanisms by which HLA polymorphism influences the immune response. These include: 1) HLA class I interactions with innate immune receptors such as KIR and LILR; 2) cell surface expression levels of HLA class I and class II; and 3) allele specific variation in HLA dependency on tapasin, a key component of the peptide loading complex. Dr. Carrington’s studies have resulted in the detection of novel HLA associations in a fairly large number of different types of human disorders including infectious diseases, cancer, autoimmunity, reproductive diseases and transplantation outcome. The lab continues to develop innovative approaches to analyze gene-disease associations by combining experimental and computational methods.

 

Dr. Carrington obtained her Ph.D. at Iowa State University in the Immunobiology Department. She performed her postdoctoral studies in the departments of Immunology and Microbiology at Duke University and the University of North Carolina. Prior to her appointment at the NCI, she was a faculty member in the Immunology Department at Duke University.

 

Lab Members

 

Selected Publications

Petersdorf, E.W., Stevenson, P., Bengtsson, M., De Santis, D., Dubois, V., Gooley, T., Horowitz, M., Hsu, K., Madrigal, J.A., Malkki, M., McKallor, C., Morishima, Y., Oudshoorn, M., Spellman, S.R., Villard, J., Carrington, M. HLA-B leader and survivorship after HLA-mismatched unrelated donor transplantation. Blood, 2020, DOI: 10.1182/blood.2020005743

 

Kulkarni, S., Lied, A., Kulkarni, V., Rucevic, M., Ewy, R., Singh, S., McLaren, P.J., Viard, M., Martin, M.P., Naranbhai, V., Zou, C., Lin, Z., Gatanaga, H., Oka, S., Takiguchi, M., Thio, C.L., Margolick, J., Kirk, G.D., Goedert, J.J., Hoots, W.K., Deeks, S.G., Haas, D.W., Michael, N., Walker, B., Le Gall, S., Chowdhury, F., Yu, X.G., and Carrington, M.: CCR5AS IncRNA variation differentially regulates CCR5, influencing viral disease outcome. Nat. Immunol., 20:824-834, 2019.

 

Martin, M.P., Naranbhai, V., Shea, P.R., Qi, Y., Ramsuran, V., Vince, N., Gao, X., Thomas, R., Brumme, Z.L., Carlson, J.M., Wolinsky, S., Goedert, J.J., Walker, B., Segal, F.P., Deeks, S., Haas, D., Migueles, S., Connors, M., Micheal, N., Fellay, J., Price, D.A., Lafont, B.A., Pymm, P., Saunders, P.M., Widjaja, J., Wong, S.C., Vivian, J.P., Rossjohn, J., Brooks, A.G., and Carrington, M.: Killer cell immunoglobulin-like receptor 3DL1 variation modifies HLA-B*57 protection against HIV-1. J. Clin. Invest., 128:1903-1912, 2018.

 

Ramsuran, V., Naranbhai, V., Horowitz, A., Qi, Y., Martin, M.P., Yuki, Y., Gao, X., Walker-Sperling, V., Del Prete, G.Q., Schneider, D.K., Lifson, J.D., Fellay, J., Deeks, S.G., Martin, J.N., Goedert, J.J., Wolinsky, S.M., Michael, N.L., Kirk, G.D., Buchbinder, S., Haas, D., Ndung’u, T., Goulder, P., Parham, P., Walker, B.D., Carlson, J.M., and Carrington, M.: Elevated HLA-A expression impairs HIV control through inhibition of NKG2A expressing cells. Science, 359:86-90, 2018.

 

Kulkarni, S., Ramsuran, V., Rucevic, Singh, S., M. Lied, A., Kulkarni, V., O’hUigin, C., Le Gail, S., and Carrington, M.: Posttranscriptional regulation of HLA-A protein expression by alternative polyadenylation signals involving the RNA-Binding protein syncrip. J. Immunol., 199:3892-3899, 2017.

 

Ramsuran, V., Hernandez-Sanchez, P.G., O’hUigin, C., Sharma, G., Spence, N., Augusto, D.G., Gao, X., Garcia-Sepulveda, C.A., Kaur, G., Mehra, N.K., and Carrington, M.: Sequence and phylogenetic analysis of the untranslated promoter regions for HLA class I genes. J. Immunol., 198:2320-2329, 2017.

 

Apps, R., Del Prete, G.Q., Chatterjee, P., Lara, A., Brumme, Z.L., Brockman, M.A., Neil, S., Pickering, S., Schneider, D.K., Piechocka-Trocha, A., Walker, B.D., Thomas, R., Shaw, G.M., Hahn, B.H., Keele, B.F., Lifson, J.D., and Carrington, M.: HIV-1 Vpu mediates HLA-C downregulation. Cell Host Microbe, 19:686-695, 2016.

 

Ramsuran, V., Kulkarni, S., O’hUigin, C., Yuki, Y., Augusto, D.G., Gao, X., and Carrington, M.: Epigenetic regulation of HLA-A allelic expression levels. Hum. Mol. Genet., 24:4268-4275, 2015.

 

Apps, R., Meng, Z., Del Prete, G.Q., Lifson, J.D., Zhou, M., and Carrington, M.: Relative expression levels of the HLA class-I proteins in normal and HIV-infected cells. J. Immunol., 194:3594-3600, 2015.

 

Bashirova, A.A., Martin-Gayo, E., Jones, D.C., Qi, Y., Apps, R., Gao, X., Burke, P.S., Taylor, C.J., Rogich, J., Wolinsky, S., Bream, J.H., Duggal, P., Hussain, S., Martinson, J., Weintrob, A., Kirk, G.D., Fellay, J., Buchbinder, S.P., Goedert, J.J., Deeks, S.G., Pereyra, F., Trowsdale, J., Lichterfeld, M., Telenti, A., Walker, B.D., Allen, R.L., Carrington, M., and Yu, X.G.: LILRB2 interaction with HLA class I correlates with control of HIV-1 infection. PLoS Genet., 10:e1004196, 2014

 

Kulkarni, S., Qi, Y., O’hUigin, C., Pereyra, F., Ramsuran, V., McLaren, P., Fellay, J., Nelson, G., Chen, H., Liao, W., Bass, S., Apps, R., Gao, X., Yuki, Y., Lied, A., Ganesan, A., Hunt, P., Deeks, S.G., Wolinsky, S., Walker, B., and Carrington, M.: Genetic interplay between HLA-C and MIR148A in HIV control and Crohn’s disease. PNAS, 110:20705-20710, 2013.

 

Apps, R., Qi, Y., Carlson, J.M., Gao, X., Thomas, R., Yuki, Y., Del Prete, G., Goulder, P., Brumme, Z.L., Brumme, C.J., John, M., Mallal, S., Nelson, G., Bosch, R., Heckerman, D., Stein, J.L., Soderberg, K.A., Moody, M.A., Denny, T.N., Moffett, A., Goedert, J.J., Buchbinder, S., Kirk, G.D., Fellay, J., McLaren, P., Deeks, S.G., Pereyra, F., Walker, B., Michael, N.L., Weintrob, A., Wolinsky, S., and Carrington, M.: Influence of HLA-C expression level on HIV control. Science, 340:87-91, 2013

 

Kulkarni, S., Savan, R., Qi, Y., Gao, X., Yuki, Y., Bass, S.E., Martin, M.P., Hunt, P., Deeks, S.G., Telenti, A., Pereyra, F., Goldstein, D., Wolinsky, S., Walker, B., Young, H.A., and Carrington, M.: Differential microRNA regulation of HLA-C expression and its association with HIV control. Nature. 472:495-498, 2011.