April 18, 17
The Ragon Institute Virology Core addresses the need for a reliable source of many HIV characterization services such as nucleic acid isolation, viral stock propagation, and viral genome sequencing. The Core gives users access to top-of-the line equipment and to the specific expertise necessary to properly design and safely execute HIV research in BSL-2+ facilities.
With an solid customer base, knowledgeable staff, and nearly a decade of experience, the Ragon Virology Core is a dependable resource for HIV characterization support. With the HIV Virology Core, researchers have a timely, reliable option for the generation of viral stocks and a variety of virus characterization methods while continuing with their own research.
Investigators may submit requests for various services, and maintain critical customization over characterization applications.
Viral RNA Isolation
Isolation of RNA from cell free fluid. Fee per sample.
Viral RNA Quantification
Determination of HIV viral load of a sample of RNA in copies/ul. Fee per sample.
Viral RNA Amplification
Amplification of select genes or regions of viral genomes (HIV, HCV, SIV, Dengue). Fee per amplicon.
Production of NL4-3 and JRCSF viruses. The final product is a vial(s) of the desired virus with titer. Fee per vial of virus.* See POLICIES regarding infectivity and titer.
Agilent Bioanalyzer 2100
Provides sizing, quantification and QC of RNA and DNA. Routinely used to assess RNA quality proper to microarray or RNA-Seq, and to assess DNA prior to NGS. Instrument use only. For kit information click here.
The Virology Core has a Fluidigm BioMark HD with two IFCs (an MX and an HX) for 48.48 or 96.96 Taqman Chemistry qPCR. For more information on this instrument, visit BioMarkHD. (EvaGreen Chemistry available upon request).
Run your own NGS libraries on the MiSeq desktop sequencer, which can generate >20M reads per run. Training required. Instrument use only. Visit Illumina’s MiSeq training and sequencing and library preparation kit pages for additional information.
Qiagen QiaCube HT
After appropriate training, users may access the Qiagen Qiacube HT which allows isolation of Viral RNA of up to 96 samples at a time. BL2+ access required. Instrument and kit information can be found here.
Sanger Sequencing (Self-Service)
After appropriate training, users have access to an AB3130XL Genetic Analyzer to run samples they have prepared themselves.
|Service||Ragon/ Partners User||External Academic User||External Industry User|
|Training for Virology Equipment||45.00||65.00||72.00|
|Viral RNA Isolation||55.00||79.00||87.00|
|Viral RNA Quantification||16.00||23.00||25.00|
|Virus Propagation, per vial||32.00||46.00||51.00|
|Fluidigm Biomark HD 96×96||905.00||1303.00||1439.00|
|Fluidigm Biomark HD 48×48||345.00||497.00||549.00|
How to Pay
Administrators of the Virology Core will handle billing responsibilities.
Billing to accounts within Partners affiliates will occur through internal funds transfers initiated by the Core. Customers will receive an invoice that they may contest within 30 days of receipt at which point the funds will be transferred. Please provide a valid fund number with all submissions.
In both Partners and non-Partners cases, it is the responsibility of the end-user investigator to ensure that the fund number s/he provides is valid.
Requests may be submitted electronically through the Red Cap submission form.
Contact Senior Lab Manager, Karen Power with any questions.
If the requestor requires any assistance, a consultation may be scheduled. Requestors are responsible for the delivery of samples to the Allen Lab (if applicable).
Certain services require the use of the BL-2+ facility. No customer is allowed entry into this facility without mandatory safety training.
Acknowledgements and Authorship
The work performed by the Ragon Virology Core should be acknowledged in papers, presentations, posters, scholarly reports and all publications. Additionally, if a Core member has made a significant contribution to your research, please consider authorship.
Virus stocks provided by the Virology Core are quality controlled for infectivity on stimulated human PBMCs and are titered on stimulated human PBMCs by standard endpoint TCID50 assay. Although these stocks are also routinely used for in vivo infection in the humanized mouse model, the stocks are not quality controlled for this use, and the Virology Core makes no guarantee as to infectivity in the humanized mouse. For uses outside of infection of stimulated human PBMC, end users of virus stocks are advised to perform their own small-scale infectivity tests prior to initiating large-scale studies.
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