Ragon Institute

The Discover S8 is a spectral flow cytometry sorter with sort-capable image analysis. See product brochure [PDF]

Located in BL2 lab 334, in a biosafety cabinet. Can be used to sort healthy and infectious human samples. Sort up to 6 populations into tubes. Sort into plates, up to 384-well. Nozzle size options: 85 micron, 100 micron, 130 micron. See tech specs [PDF]

• Nickname “S8“
• 5 lasers, 78 detectors for full spectral analysis
• 3 blue laser scatter detectors: forward scatter, side scatter, and axial light loss
• 3 fluorescence imaging channels, all excited by the 488 nm blue laser
• Imaging channels: FL1 (534/46), FL2 (600/60), FL3 (788/225)
• Please note: No compensation or unmixing available for fluorescence imaging channels
• Use the BD Spectrum Viewer for panel design

Two Aria Fusion cell sorters, nicknamed the “SORP” and the “Fusion.” Both located in BL2 lab 334, in biosafety cabinets. Can be used to sort healthy and infectious human samples. Sort up to 4 populations into tubes. Sort into any type of plate, up to 384-well. Nozzle size options: 70 micron, 85 micron, 100 micron, 130 micron.

We provide full-service assisted cell sorting on the Aria Fusion cell sorters. We can also train you to sort on your own if you choose to do so.

5-Laser SORP Fusion

• Nickname “SORP“
• 5 lasers, 18 fluorescence parameters

4-Laser Fusion

• Nickname “Fusion“
• 4 lasers, 16 fluorescence parameters

Two Sony SH800 cell sorters, nicknamed the “BL2 Sony” and the “Yeast Sony.” Disposable nozzle chip size options: 70 micron, 100 micron, 130 micron.

Both SH800 cell sorters are fully self-run instruments. We provide user training.

BL2 Sony SH800

• Nickname “BL2 Sony“
• 4 co-linear lasers, 6 fluorescence parameters
• Sort up to 2-way into tubes
• Sort into plates
• Located in BL2 lab 334, in a biosafety cabinet
• Sort mammalian cells, including human samples

Yeast Sony SH800

• Nickname “Yeast Sony“
• 3 co-linear lasers, 6 fluorescence parameters
• Sort up to 2-way into tubes
• Plate sorting not available
• BL1 sorting only: no unfixed human samples
• Intended for sorting yeast

The 5-Laser Cytek Aurora is a spectral flow cytometry analyzer. With your free account on Cytek Cloud, experiments can be created and imported into SpectroFlo software. If you are new to spectral cytometry, view the short videos on SpectroLearn to find out how to build and optimize a spectral cytometry panel and validate your reference controls and unmixing.

Optical configuration and fluorochrome guide [PDF]

• 5 lasers, 64 detectors for full spectral analysis
• Volumetric: no need for counting beads
• Autofluorescence Explorer allows unmixing of multiple autofluorescence signals in a single sample
• Daily QC beads automatically normalize detector gains for reproducibility across time and across instruments
• Load samples in tubes, or use the auto-loader for tube racks and plates
• Deep-well plates ok

The ImageStream is a true imaging flow cytometer. You can think of it as a very fast quantitative high-throughput microscope, or as a flow cytometer that takes brightfield and fluorescence images of all events.

See product brochure [PDF]

Sample preparation and quick guides with optical configuration [PDF]

• 4 lasers, max. 10 fluorescence parameters
• Detectors: 2 cameras, each with 6 channels
• Objectives: 20x, 40x, 60x
• Max resolution: 0.3 micron x 0.3 micron pixel size
• EDF option provides enhanced depth of field for spot counting
• Compensation removes observed spectral overlap between fluorescence channels
• Data is collected as .fcs (flow cytometry standard) as well as a proprietary image data format
• Image analysis software IDEAS 6.2 is available for free and may be installed on any Windows PC

Cytometry!

Cytometry!

Cytometry!

Cytometry!

Cytometry!