December 19, 16
Ragon Imaging Core
The Ragon Institute Flow Cytometry core provides access to a 2 laser BD FACSCalibur, a 3 laser BD LSR2, two 4 laser BD LSR2s, and the first 5 laser BD LSR Fortessa in the Boston area, as well as a 5 laser, 18 color high speed cell sorter (BD FACSAria SORP) that allows researchers to separate and collect populations of interest for further study. In addition, the sorter is housed in a biosafety cabinet to allow for sorting of infectious samples.
The Ragon Imaging Core microscopy facility provides training and access to six imaging systems, including a Zeiss LSM510 laser scanning confocal microscope, a fully automated Zeiss Axio Observer microscope (both in BL2+ facilities), two slide scanning systems (MIRAX MIDI and TissueFAXS) for automated screening of cells populations in tissue sections, a manual Zeiss A1 fluorescence/phase contrast microscope, and the ImageStreamX MkII, an imaging flow cytometer. Consultation is also provided on imaging applications and image analysis.
Mass cytometry combines the advantages of single cell high speed analysis common to conventional flow cytometry with the ability to resolve up to 40 metal probes with minimal signal overlap common to atomic mass spectroscopy. Therefore, mass cytometry enables comprehensive profiling of cellular phenotype, signaling state, cytokine/chemokine expression, and viability.
Human Immune System Mouse Program
The Ragon Institute Human Immune System Mouse Program was established in order to further develop an ideal animal model to study HIV pathogenesis and test vaccine efficacy and to make it available to the wider Ragon research community. Highly experienced personnel produce these animals and perform experiments in collaboration with Ragon investigators at very reasonable cost.
As part of the Ragon Institute’s overall goal to study the pathogenesis of HIV and develop effective vaccine approaches, the Virology Platform provides critical infrastructure to provide a centralized facility for preparation, titreing, and storage of HIV stocks for immunological and virological studies. We also offer viral sequencing services and the evaluation of viral replication capacity of HIV strains and constructs.
The Ragon Tissue Platform has been established to provide the necessary infrastructure to conduct patient-based research investigating tissue specific immune responses against HIV. We seek to significantly reduce the major obstacle to initiating tissue based studies by providing access to human tissue specimens to assist in studies of HIV pathogenesis and tissue-specific immune responses against HIV.
Biostatistics serves the needs of the HIV/AIDS researchers within the Ragon Institute and its affiliates. In particular, members of the Biostatistics Core provide expertise in the planning, conduct and analysis of research with the goal of enhancing the scientific quality of HIV-related research at the institute. The primary objective of the core is to ensure that studies are well designed, correctly analyzed, clearly presented, and correctly interpreted.
Biosafety Level 3 (BL3)
The BL3 Core provides training and consultation for members of the HU CFAR community who are planning TB related projects and provides access to BSL-3 tissue culture, live cell sorting, and wide-field live cell videomicroscopy.Learn More
The Processing Lab isolates blood plasma, DNA, and peripheral blood mononuclear cells from HIV positive and negative samples, making specimens for use during experiments.Learn More
Cellular Immunology Database (CIDB)
The Ragon Institute Cellular Immunology Database (CIDB) is a Cache InterSystems database managed with a cross platform User Interface developed using the Adobe Flex framework. The database houses genetic and clinical host information, as well as immunological and virological data pertaining to the study of HIV/AIDS and efforts to develop a successful HIV vaccine.